** Answer:** The effectiveness of the pesticide is not decreased rather the spiders body became resistant to the pesticide. When a pesticide is used on a large population of spiders some of them may survive due to the their distinct genetic makeup. The spiders that were not having this distinct gene died. The individuals that were having distinct genetic makeup survived and reproduce and hence their number increased, their population increased and hence the resistant spiders increased so, next time the application killed only 45% of the population rather 78%.
Evolution of pesticide resistance population of spiders ;
The decrease in the effectiveness of the pesticide is largely due to the development of pesticide resistance among the spider population. After the first application, surviving spiders likely possessed resistance traits which were passed on to their offspring. As a result, by the second application, the population was predominantly resistant, leading to only 45% being killed by the pesticide.
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