Why did the heat-treated enzyme behave differently than the non-heated enzyme?

Heat can affect the three-dimensional shape of a protein (called tertiary structure). Enzymes are proteins, and they can only function properly within a certain temperature and pH range. A temperature different than their ideal temperature can make them less efficient or even prevent them from working at all.

Answered by ljndr | 2024-06-10

The heat-treated enzyme behaves differently than the non-heated enzyme due to denaturation, which alters its three-dimensional structure and affects its ability to bind substrates. This denaturation occurs because enzymes are sensitive to temperature changes, with high temperatures disrupting the interactions that hold their structure together. In some cases, enzymes may regain some activity if cooled down, allowing them to re-fold under suitable conditions.
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Answered by Anonymous | 2024-12-26